Days Set Up
Monday - Friday
Analytic Time

2 - 3 days

MiChart Code
Soft Order Code

Test Updated:


AFP, Maternal Serum
Maternal Serum AFP
Neural tube defects (NTD) Risk

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Find a Requisition

All specimens should be accompanied by a requisition.

Submitting Specimens

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Order Kits and Supplies

MLabs provides all the supplies necessary for the collection of specimens.

Test FAQ

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Test Overview

Test Methodology

Chemiluminescent Immunoassay (CLIA)

Test Usage

To aid in the detection of fetal neural tube defects (NTD).

Reference Range

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

Collect specimen in a red top tube from a patient with a maternal gestation of between 16 weeks 0 days and 22 weeks 6 days. A completed Warde Medical Laboratory Prenatal Information Sheet must accompany the specimen.

Collection Offsite

Collect specimen in a red top tube from a patient with a maternal gestation of between 16 weeks 0 days and 22 weeks 6 days. Centrifuge, aliquot serum into a plastic vial within 2 hours of collection and refrigerate. Please provide a completed QUAD/AFP Requisition available online at or the following information: gestational age (weeks and days on first ultrasound date >6 weeks 0 days; if ultrasound information is not available record first days of last menstrual period), maternal weight, maternal date of birth, insulin-dependent diabetes status prior to pregnancy, multiple gestation (single, twin, triplets), race, and previous history of Down syndrome or neural tube defect (NTD) pregnancy for the patient.

Red Top Tube
Normal Volume
1 mL serum
Minimum Volume
0.6 mL serum

Billing Information

CPT Code
Pro Fee Code

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.