Histocompatibility

Director:                                                                                    
Daniel Ramon, Ph.D., D[ABHI], HCLD[ABB]
                                                                
General Supervisor:
Cynthia Schall, BS, CHS(ABHI)
                                               
Supervisor, Serology/Flow: 
Timothy William, MBA
Supervisor, Molecular: 
Kathryn Daavettila, CHT, MT(ASCP)


Services:
The Histocompatibility Laboratory offers a full range of tissue typing, or histocompatibility procedures to support the needs of our University of Michigan patients as well as our clients across the country.

Using the most current technology and assays, our mission is to provide a quality service to the University of Michigan Health System’s Hematopoietic Cell Transplant Program and the Solid Organ Transplant Center, one of the largest in the nation.

For the Hematopoietic Cell Transplant program, we reach a high resolution HLA typing for donor and recipient utilizing Sequence Based Typing (SBT) technique as well as Sequence Specific Primer or Oligonucleotide Probes molecular reactions (SSP, SSO). For those recipients with less than ideal donors, the laboratory tests for anti-HLA antibodies directed against the mismatched antigens to help the transplant team in the risk assessment of engraftment delay or graft rejection.

The Histocompatibility Laboratory collaborates with the Solid Organ Transplant Team in the pre and post-transplant immunological risk assessment to avoid graft rejection and to monitor treatment. Patients and candidate donors are typed for HLA molecules by DNA methods. In addition, the presence or absence of anti HLA antibodies (PRA%) is monitored by high sensitivity and specificity solid phase assays (Luminex). The final donor selection is supported by the Lymphocytes Flow Cytometric Crossmatch assay. For those individuals that are highly sensitized patients as well as pre-transplant patients, the laboratory also performs an endothelial precursor cells crossmatch and MICA antibody screening.

The Histocompatibility Laboratory performs HLA typing for disease association studies such as Narcolepsy (HLA-DQB1*0602), Uveitis (HLA-B27), Ankylosing Spondylitis (HLA-B27)  and Bird Shot Retinopathy (HLA-A29) as well as others. In addition, HLA typing and anti-HLA antibody screening is performed for transfusion support services.

The Histocompatibility Laboratory is certified by the American Society of Histocompatibility and Immunogenetics (ASHI) and the College of American Pathologists (CAP).

Please refer to the MLabs Test Catalog for available assays and specimen collection requirements.     

The Histocompatibility Laboratory offers the following assays:

Solid Organ Transplant (Kidney, Heart, Lung, Liver and Pancreas) 

Hematopoeitic Cell Transplant

HLA Typing & PRA for Transfusion Support or Platelets

Disease Association Studies


More information: